Table of Contents

Biological Sciences

• Agricultural Sciences

  • Open Access

    Canine olfactory detection of a vectored phytobacterial pathogen, Liberibacter asiaticus, and integration with disease control

    • Video

    Timothy Gottwald, Gavin Poole, Thomas McCollum, David Hall, John Hartung, Jinhe Bai, Weiqi Luo, Drew Posny, Yong-Ping Duan, Earl Taylor, John da Graça, MaryLou Polek, Frank Louws, and William Schneider

    PNAS February 18, 2020 117 (7) 3492-3501; first published February 3, 2020. https://doi.org/10.1073/pnas.1914296117

• Applied Biological Sciences

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    High-throughput quantitative microscopy-based half-life measurements of intravenously injected agents

    Laura G. Bracaglia, Alexandra S. Piotrowski-Daspit, Chun-Yu Lin, Zoe M. Moscato, Yongheng Wang, Gregory T. Tietjen, and W. Mark Saltzman

    PNAS February 18, 2020 117 (7) 3502-3508; first published February 3, 2020. https://doi.org/10.1073/pnas.1915450117

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    Real-time particle-by-particle detection of erythrocyte-camouflaged microsensor with extended circulation time in the bloodstream

    Wenjun Di, Xuefei Tan, Isen Andrew C. Calderon, Ashlyn E. Neal Reilly, Mark Niedre, and Heather A. Clark

    PNAS February 18, 2020 117 (7) 3509-3517; first published February 4, 2020. https://doi.org/10.1073/pnas.1914913117

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    Spatiotemporal dynamic monitoring of fatty acid–receptor interaction on single living cells by multiplexed Raman imaging

    Wei Zhang, Fangjun Lin, Yan Liu, Han Zhang, Timothy A. Gilbertson, and Anhong Zhou

    PNAS February 18, 2020 117 (7) 3518-3527; first published February 3, 2020. https://doi.org/10.1073/pnas.1916238117

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• Biochemistry

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    Synonymous codon substitutions perturb cotranslational protein folding in vivo and impair cell fitness

    Ian M. Walsh, Micayla A. Bowman, Iker F. Soto Santarriaga, Anabel Rodriguez, and Patricia L. Clark

    PNAS February 18, 2020 117 (7) 3528-3534; first published February 3, 2020. https://doi.org/10.1073/pnas.1907126117

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  • Open Access

    Human MutLγ, the MLH1–MLH3 heterodimer, is an endonuclease that promotes DNA expansion

    Lyudmila Y. Kadyrova, Vaibhavi Gujar, Vickers Burdett, Paul L. Modrich, and Farid A. Kadyrov

    PNAS February 18, 2020 117 (7) 3535-3542; first published February 3, 2020. https://doi.org/10.1073/pnas.1914718117
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    Role of a DEF/Y motif in histone H2A-H2B recognition and nucleosome editing

    Yan Huang, Lu Sun, Leonidas Pierrakeas, Linchang Dai, Lu Pan, Ed Luk, and Zheng Zhou

    PNAS February 18, 2020 117 (7) 3543-3550; first published January 30, 2020. https://doi.org/10.1073/pnas.1914313117

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    Unique subsite specificity and potential natural function of a chitosan deacetylase from the human pathogen Cryptococcus neoformans

    Lea Hembach, Martin Bonin, Christian Gorzelanny, and Bruno M. Moerschbacher

    PNAS February 18, 2020 117 (7) 3551-3559; first published February 3, 2020. https://doi.org/10.1073/pnas.1915798117

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    Np₄A alarmones function in bacteria as precursors to RNA caps

    • From the Cover

    Daniel J. Luciano and Joel G. Belasco

    PNAS February 18, 2020 117 (7) 3560-3567; first published February 4, 2020. https://doi.org/10.1073/pnas.1914229117

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    See companion article:

    • Deciphering the RNA capping process in bacteria

      - Mar 03, 2020
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    Control of XPR1-dependent cellular phosphate efflux by InsP₈ is an exemplar for functionally-exclusive inositol pyrophosphate signaling

    Xingyao Li, Chunfang Gu, Sarah Hostachy, Soumyadip Sahu, Christopher Wittwer, Henning J. Jessen, Dorothea Fiedler, Huanchen Wang, and Stephen B. Shears

    PNAS February 18, 2020 117 (7) 3568-3574; first published February 4, 2020. https://doi.org/10.1073/pnas.1908830117

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• Biophysics and Computational Biology

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    Energy dissipation bounds for autonomous thermodynamic cycles

    Samuel J. Bryant and Benjamin B. Machta

    PNAS February 18, 2020 117 (7) 3478-3483; first published February 4, 2020. https://doi.org/10.1073/pnas.1915676117

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  • Open Access

    Dynamic clamp constructed phase diagram for the Hodgkin and Huxley model of excitability

    Hillel Ori, Hananel Hazan, Eve Marder, and Shimon Marom

    PNAS February 18, 2020 117 (7) 3575-3582; first published February 5, 2020. https://doi.org/10.1073/pnas.1916514117
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    M2 amphipathic helices facilitate pH-dependent conformational transition in influenza A virus

    • Video

    Hedieh Torabifard, Afra Panahi, and Charles L. Brooks III

    PNAS February 18, 2020 117 (7) 3583-3591; first published February 3, 2020. https://doi.org/10.1073/pnas.1913385117

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    The α-synuclein hereditary mutation E46K unlocks a more stable, pathogenic fibril structure

    David R. Boyer, Binsen Li, Chuanqi Sun, Weijia Fan, Kang Zhou, Michael P. Hughes, Michael R. Sawaya, Lin Jiang, and David S. Eisenberg

    PNAS February 18, 2020 117 (7) 3592-3602; first published February 3, 2020. https://doi.org/10.1073/pnas.1917914117

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    Drosophila YBX1 homolog YPS promotes ovarian germ line stem cell development by preferentially recognizing 5-methylcytosine RNAs

    Fan Zou, Renjun Tu, Bo Duan, Zhenlin Yang, Zhaohua Ping, Xiaoqing Song, Shiyuan Chen, Andrew Price, Hua Li, Allison Scott, Anoja Perera, Sisi Li, and Ting Xie

    PNAS February 18, 2020 117 (7) 3603-3609; first published February 3, 2020. https://doi.org/10.1073/pnas.1910862117

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  • Open Access

    Elongation factor-Tu can repetitively engage aminoacyl-tRNA within the ribosome during the proofreading stage of tRNA selection

    Justin C. Morse, Dylan Girodat, Benjamin J. Burnett, Mikael Holm, Roger B. Altman, Karissa Y. Sanbonmatsu, Hans-Joachim Wieden, and Scott C. Blanchard

    PNAS February 18, 2020 117 (7) 3610-3620; first published February 5, 2020. https://doi.org/10.1073/pnas.1904469117

• Cell Biology

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    Selective targeting of TET catalytic domain promotes somatic cell reprogramming

    Anup Kumar Singh, Bo Zhao, Xiuhua Liu, Xin Wang, Hongzhi Li, Hanjun Qin, Xiwei Wu, Yuelong Ma, David Horne, and Xiaochun Yu

    PNAS February 18, 2020 117 (7) 3621-3626; first published February 5, 2020. https://doi.org/10.1073/pnas.1910702117

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    Splice switching an oncogenic ratio of SmgGDS isoforms as a strategy to diminish malignancy

    Anthony C. Brandt, Lisa McNally, Ellen L. Lorimer, Bethany Unger, Olivia J. Koehn, Kiall F. Suazo, Lisa Rein, Aniko Szabo, Shirng-Wern Tsaih, Mark D. Distefano, Michael J. Flister, Frank Rigo, Mark T. McNally, and Carol L. Williams

    PNAS February 18, 2020 117 (7) 3627-3636; first published February 4, 2020. https://doi.org/10.1073/pnas.1914153117

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  • Open Access

    TIP5 primes prostate luminal cells for the oncogenic transformation mediated by PTEN-loss

    Karolina Pietrzak, Rostyslav Kuzyakiv, Ronald Simon, Marco Bolis, Dominik Bär, Rossana Aprigliano, Jean-Philippe Theurillat, Guido Sauter, and Raffaella Santoro

    PNAS February 18, 2020 117 (7) 3637-3647; first published February 5, 2020. https://doi.org/10.1073/pnas.1911673117

• Ecology

  • Open Access

    Impacts of current and future large dams on the geographic range connectivity of freshwater fish worldwide

    Valerio Barbarossa, Rafael J. P. Schmitt, Mark A. J. Huijbregts, Christiane Zarfl, Henry King, and Aafke M. Schipper

    PNAS February 18, 2020 117 (7) 3648-3655; first published February 3, 2020. https://doi.org/10.1073/pnas.1912776117
  • Open Access

    Ecological drivers of bacterial community assembly in synthetic phycospheres

    He Fu, Mario Uchimiya, Jeff Gore, and Mary Ann Moran

    PNAS February 18, 2020 117 (7) 3656-3662; first published February 3, 2020. https://doi.org/10.1073/pnas.1917265117
  • Open Access

    Mismatches between demographic niches and geographic distributions are strongest in poorly dispersed and highly persistent plant species

    • From the Cover

    Jörn Pagel, Martina Treurnicht, William J. Bond, Tineke Kraaij, Henning Nottebrock, AnneLise Schutte-Vlok, Jeanne Tonnabel, Karen J. Esler, and Frank M. Schurr

    PNAS February 18, 2020 117 (7) 3663-3669; first published February 6, 2020. https://doi.org/10.1073/pnas.1908684117

• Environmental Sciences

  • Open Access

    Shale gas development has limited effects on stream biology and geochemistry in a gradient-based, multiparameter study in Pennsylvania

    Adam C. Mumford, Kelly O. Maloney, Denise M. Akob, Sarah Nettemann, Arianne Proctor, Jason Ditty, Luke Ulsamer, Josh Lookenbill, and Isabelle M. Cozzarelli

    PNAS February 18, 2020 117 (7) 3670-3677; first published February 3, 2020. https://doi.org/10.1073/pnas.1911458117

• Evolution

  • Open Access

    Whole-proteome tree of life suggests a deep burst of organism diversity

    JaeJin Choi and Sung-Hou Kim

    PNAS February 18, 2020 117 (7) 3678-3686; first published February 4, 2020. https://doi.org/10.1073/pnas.1915766117

• Immunology and Inflammation

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    Transdermal cold atmospheric plasma-mediated immune checkpoint blockade therapy

    • Video

    Guojun Chen, Zhitong Chen, Di Wen, Zejun Wang, Hongjun Li, Yi Zeng, Gianpietro Dotti, Richard E. Wirz, and Zhen Gu

    PNAS February 18, 2020 117 (7) 3687-3692; first published February 6, 2020. https://doi.org/10.1073/pnas.1917891117

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    Glioblastomas exploit truncated O-linked glycans for local and distant immune modulation via the macrophage galactose-type lectin

    Sophie A. Dusoswa, Jan Verhoeff, Erik Abels, Santiago P. Méndez-Huergo, Diego O. Croci, Lisan H. Kuijper, Elena de Miguel, Valerie M. C. J. Wouters, Myron G. Best, Ernesto Rodriguez, Lenneke A. M. Cornelissen, Sandra J. van Vliet, Pieter Wesseling, Xandra O. Breakefield, David P. Noske, Thomas Würdinger, Marike L. D. Broekman, Gabriel A. Rabinovich, Yvette van Kooyk, and Juan J. Garcia-Vallejo

    PNAS February 18, 2020 117 (7) 3693-3703; first published February 4, 2020. https://doi.org/10.1073/pnas.1907921117

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  • Open Access

    Defective HIV-1 proviruses produce viral proteins

    Hiromi Imamichi, Mindy Smith, Joseph W. Adelsberger, Taisuke Izumi, Francesca Scrimieri, Brad T. Sherman, Catherine A. Rehm, Tomozumi Imamichi, Alice Pau, Marta Catalfamo, Anthony S. Fauci, and H. Clifford Lane

    PNAS February 18, 2020 117 (7) 3704-3710; first published February 6, 2020. https://doi.org/10.1073/pnas.1917876117
  • Open Access

    Activation of mosquito immunity blocks the development of transmission-stage filarial nematodes

    Elizabeth B. Edgerton, Abigail R. McCrea, Corbett T. Berry, Jenny Y. Kwok, Letitia K. Thompson, Brittany Watson, Elizabeth M. Fuller, Thomas J. Nolan, James B. Lok, and Michael Povelones

    PNAS February 18, 2020 117 (7) 3711-3717; first published February 3, 2020. https://doi.org/10.1073/pnas.1909369117
  • Open Access

    An ontogenetic switch drives the positive and negative selection of B cells

    Xijin Xu, Mukta Deobagkar-Lele, Katherine R. Bull, Tanya L. Crockford, Adam J. Mead, Adam P. Cribbs, David Sims, Consuelo Anzilotti, and Richard J. Cornall

    PNAS February 18, 2020 117 (7) 3718-3727; first published February 4, 2020. https://doi.org/10.1073/pnas.1915247117

• Medical Sciences

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    All-digital histopathology by infrared-optical hybrid microscopy

    Martin Schnell, Shachi Mittal, Kianoush Falahkheirkhah, Anirudh Mittal, Kevin Yeh, Seth Kenkel, Andre Kajdacsy-Balla, P. Scott Carney, and Rohit Bhargava

    PNAS February 18, 2020 117 (7) 3388-3396; first published February 3, 2020. https://doi.org/10.1073/pnas.1912400117

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  • Open Access

    Combining microenvironment normalization strategies to improve cancer immunotherapy

    Fotios Mpekris, Chrysovalantis Voutouri, James W. Baish, Dan G. Duda, Lance L. Munn, Triantafyllos Stylianopoulos, and Rakesh K. Jain

    PNAS February 18, 2020 117 (7) 3728-3737; first published February 3, 2020. https://doi.org/10.1073/pnas.1919764117

    See companion article:

    • Immunoactivating the tumor microenvironment enhances immunotherapy as predicted by integrative computational model

      - Mar 03, 2020
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    NAD deficiency due to environmental factors or gene–environment interactions causes congenital malformations and miscarriage in mice

    • From the Cover

    Hartmut Cuny, Melissa Rapadas, Jessica Gereis, Ella M. M. A. Martin, Rosemary B. Kirk, Hongjun Shi, and Sally L. Dunwoodie

    PNAS February 18, 2020 117 (7) 3738-3747; first published February 3, 2020. https://doi.org/10.1073/pnas.1916588117

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    • Dietary modification, penetrance, and the origins of congenital malformation

      - Mar 10, 2020
  • Open Access

    Hsp47 promotes cancer metastasis by enhancing collagen-dependent cancer cell-platelet interaction

    Gaofeng Xiong, Jie Chen, Guoying Zhang, Shike Wang, Kunito Kawasaki, Jieqing Zhu, Yan Zhang, Kazuhiro Nagata, Zhenyu Li, Binhua P. Zhou, and Ren Xu

    PNAS February 18, 2020 117 (7) 3748-3758; first published February 3, 2020. https://doi.org/10.1073/pnas.1911951117

• Microbiology

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    Repair of a previously uncharacterized second host-range gene contributes to full replication of modified vaccinia virus Ankara (MVA) in human cells

    Chen Peng and Bernard Moss

    PNAS February 18, 2020 117 (7) 3759-3767; first published February 4, 2020. https://doi.org/10.1073/pnas.1921098117

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    Development of an antibody cocktail for treatment of Sudan virus infection

    Andrew S. Herbert, Jeffery W. Froude, Ramon A. Ortiz, Ana I. Kuehne, Danielle E. Dorosky, Russell R. Bakken, Samantha E. Zak, Nicole M. Josleyn, Konstantin Musiychuk, R. Mark Jones, Brian Green, Stephen J. Streatfield, Anna Z. Wec, Natasha Bohorova, Ognian Bohorov, Do H. Kim, Michael H. Pauly, Jesus Velasco, Kevin J. Whaley, Spencer W. Stonier, Zachary A. Bornholdt, Kartik Chandran, Larry Zeitlin, Darryl Sampey, Vidadi Yusibov, and John M. Dye

    PNAS February 18, 2020 117 (7) 3768-3778; first published February 3, 2020. https://doi.org/10.1073/pnas.1914985117

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    Facilitative and synergistic interactions between fungal and plant viruses

    Ruiling Bian, Ida Bagus Andika, Tianxing Pang, Ziqian Lian, Shuang Wei, Erbo Niu, Yunfeng Wu, Hideki Kondo, Xili Liu, and Liying Sun

    PNAS February 18, 2020 117 (7) 3779-3788; first published February 3, 2020. https://doi.org/10.1073/pnas.1915996117

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    Listeria monocytogenes exploits host exocytosis to promote cell-to-cell spread

    Georgina C. Dowd, Roman Mortuza, Manmeet Bhalla, Hoan Van Ngo, Yang Li, Luciano A. Rigano, and Keith Ireton

    PNAS February 18, 2020 117 (7) 3789-3796; first published February 3, 2020. https://doi.org/10.1073/pnas.1916676117

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• Neuroscience

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    Functional connectivity predicts changes in attention observed across minutes, days, and months

    Monica D. Rosenberg, Dustin Scheinost, Abigail S. Greene, Emily W. Avery, Young Hye Kwon, Emily S. Finn, Ramachandran Ramani, Maolin Qiu, R. Todd Constable, and Marvin M. Chun

    PNAS February 18, 2020 117 (7) 3797-3807; first published February 4, 2020. https://doi.org/10.1073/pnas.1912226117

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    Individual-specific functional connectivity of the amygdala: A substrate for precision psychiatry

    • From the Cover

    Chad M. Sylvester, Qiongru Yu, A. Benjamin Srivastava, Scott Marek, Annie Zheng, Dimitrios Alexopoulos, Christopher D. Smyser, Joshua S. Shimony, Mario Ortega, Donna L. Dierker, Gaurav H. Patel, Steven M. Nelson, Adrian W. Gilmore, Kathleen B. McDermott, Jeffrey J. Berg, Andrew T. Drysdale, Michael T. Perino, Abraham Z. Snyder, Ryan V. Raut, Timothy O. Laumann, Evan M. Gordon, Deanna M. Barch, Cynthia E. Rogers, Deanna J. Greene, Marcus E. Raichle, and Nico U. F. Dosenbach

    PNAS February 18, 2020 117 (7) 3808-3818; first published February 3, 2020. https://doi.org/10.1073/pnas.1910842117

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    See companion article:

    • A new vista in psychiatric treatment: Using individualized functional connectivity to track symptoms

      - Mar 03, 2020
  • Open Access

    Synaptotagmin 1 oligomers clamp and regulate different modes of neurotransmitter release

    • Video

    Erica Tagliatti, Oscar D. Bello, Philipe R. F. Mendonça, Dimitrios Kotzadimitriou, Elizabeth Nicholson, Jeff Coleman, Yulia Timofeeva, James E. Rothman, Shyam S. Krishnakumar, and Kirill E. Volynski

    PNAS February 18, 2020 117 (7) 3819-3827; first published February 3, 2020. https://doi.org/10.1073/pnas.1920403117
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    Protection of cochlear synapses from noise-induced excitotoxic trauma by blockade of Ca²⁺-permeable AMPA receptors

    Ning Hu, Mark A. Rutherford, and Steven H. Green

    PNAS February 18, 2020 117 (7) 3828-3838; first published February 3, 2020. https://doi.org/10.1073/pnas.1914247117

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    Conformational spread and dynamics in allostery of NMDA receptors

    Ryan J. Durham, Nabina Paudyal, Elisa Carrillo, Nidhi Kaur Bhatia, David M. Maclean, Vladimir Berka, Drew M. Dolino, Alemayehu A. Gorfe, and Vasanthi Jayaraman

    PNAS February 18, 2020 117 (7) 3839-3847; first published February 3, 2020. https://doi.org/10.1073/pnas.1910950117

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• Pharmacology

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    Positive allosteric modulation of indoleamine 2,3-dioxygenase 1 restrains neuroinflammation

    Giada Mondanelli, Alice Coletti, Francesco Antonio Greco, Maria Teresa Pallotta, Ciriana Orabona, Alberta Iacono, Maria Laura Belladonna, Elisa Albini, Eleonora Panfili, Francesca Fallarino, Marco Gargaro, Giorgia Manni, Davide Matino, Agostinho Carvalho, Cristina Cunha, Patricia Maciel, Massimiliano Di Filippo, Lorenzo Gaetani, Roberta Bianchi, Carmine Vacca, Ioana Maria Iamandii, Elisa Proietti, Francesca Boscia, Lucio Annunziato, Maikel Peppelenbosch, Paolo Puccetti, Paolo Calabresi, Antonio Macchiarulo, Laura Santambrogio, Claudia Volpi, and Ursula Grohmann

    PNAS February 18, 2020 117 (7) 3848-3857; first published February 5, 2020. https://doi.org/10.1073/pnas.1918215117

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• Physiology

  • Open Access

    Kv2.1 channels play opposing roles in regulating membrane potential, Ca²⁺ channel function, and myogenic tone in arterial smooth muscle

    Samantha C. O’Dwyer, Stephanie Palacio, Collin Matsumoto, Laura Guarina, Nicholas R. Klug, Sendoa Tajada, Barbara Rosati, David McKinnon, James S. Trimmer, and L. Fernando Santana

    PNAS February 18, 2020 117 (7) 3858-3866; first published February 3, 2020. https://doi.org/10.1073/pnas.1917879117

• Plant Biology

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    The versatile functions of OsALDH2B1 provide a genic basis for growth–defense trade-offs in rice

    Yinggen Ke, Meng Yuan, Hongbo Liu, Shugang Hui, Xiaofeng Qin, Jie Chen, Qinglu Zhang, Xianghua Li, Jinghua Xiao, Qifa Zhang, and Shiping Wang

    PNAS February 18, 2020 117 (7) 3867-3873; first published February 5, 2020. https://doi.org/10.1073/pnas.1918994117

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    Rhizosphere microbiome mediates systemic root metabolite exudation by root-to-root signaling

    Elisa Korenblum, Yonghui Dong, Jedrzej Szymanski, Sayantan Panda, Adam Jozwiak, Hassan Massalha, Sagit Meir, Ilana Rogachev, and Asaph Aharoni

    PNAS February 18, 2020 117 (7) 3874-3883; first published February 3, 2020. https://doi.org/10.1073/pnas.1912130117

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